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For the wound healing assay, cells were seeded the day before and scratched performed using the WoundMaker TM instrument (essenBiosience)

Extensive investigations of G6PD from G6PD-deficient cells, mostly carried out before the sequence of G6PD was known, revealed that (1) enzyme activity, even when severely reduced (sometimes to less than 1% of normal), is never completely absent and (2) enzymic properties ( K m , K i , activity on substrate analogues, and thermostability, for example) are often different from those of the normal enzyme (i.e., G6PD deficiency is associated with qualitative abnormalities)

[DOI] [PMC free article] [PubMed] [Google Scholar] Nolan RA, Reeb KL, Rong Y, Matt SM, Johnson HS, Runner K, Gaskill PJ (2020) Dopamine activates NF-kappaB and primes the NLRP3 inflammasome in primary human macrophages