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(A-D) 1.5-fold stage embryos expressing GFP::GLO-1(D132A) were stained with antibodies to PGP-2 and LMP-1

any cloudiness or visible particles indicates degradation or contamination

CHO-K1 cells were pretreated with MCD at 37C for 1 h to deplete cholesterol from cells

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is supported by NIH grants GM094584, GM094622, and GM098248