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Subsequently, according to the method described in the previous study ( 2.7 Immunofluorescence (IF) After transcardial perfusion in mice, brain tissues were harvested and pretreated with gradient dehydration in 20% and 30% sucrose solutions at 4C (to reduce ice crystal damage), then embedded in optimal cutting temperature compound and sectioned for 20 m thickness using cryostat

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[DOI] [PMC free article] [PubMed] [Google Scholar] [27].Harris JM, Chess RB, Effect of pegylation on pharmaceuticals, Nature Reviews Drug Discovery, 2 (2003) 214221

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