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GSH assay fluorescence was determined with a fluorescence spectrophotometer at 420 nm (excitation at 350 nm) after incubating 0.2 ml of supernatant with 1.7 ml of potassium phosphate-EDTA buffer (pH 8.0) and 0.1 ml of fluorescence reagent o -phthaldialdehyde (1 mg/ml) for 15 min

Critical review of exposure and effects: implications for setting regulatory health criteria for ingested copper

Young IS, Woodside JV

These results indicated that tumors with high ACTL6A expression, which rely more heavily on GSH metabolism than those with low ACTL6A expression, are more sensitive to ferroptosis inducers or GCLC inhibitors