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Acknowledgments The authors thank Dimitry Lapage for his guidance on animal feeding experiment and Anneke Ovyn for her guidance on the parameter test in the laboratory

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doi: 10.1016/j.ecoenv.2022.113238 104 Silva-GarcaOValdez-AlarcnJJBaizabal-AguirreVM

Rather than elute the protein with glutathione buffer, a PBS solution containing the enzyme is loaded onto the column and incubated for several hours at room temperature (4 C for PreScission protease)

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