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Lu X, Paliogiannis P, Calvisi DF, Chen X

Cellular crowding imposes global constraints on the chemistry and evolution of proteomes
Interestingly, NOX4-deficient mice are completely protected against bleomycin-induced AT2 cell apoptosis and pulmonary fibrosis, but not against bleomycin-induced pulmonary inflammation [25]

ROS and GSH/GSSG ratio quantification Cells were incubated in a 96-well plate with 10M H2-DCFDA (Life Technology, D399) for 45 minutes in serum-free media to detect total intracellular ROS

Microsomal cytochrome P450 enzymes (notably CYP2E1 in alcohol metabolism): These generate ROS as part of detoxification reactions and sometimes uncoupled electron-leak events

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