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DNA probes used for EMSA were obtained by annealing complementary oligos (EMSA copB-F and -R, Supplementary Table S2), which were biotin-labeled using the Biotin 3-End DNA Labeling Kit (Thermo Scientific)
With practice, most people find their technique improves and discomfort decreases

doi: 10.1182/bloodadvances.2019000975 198 HisadaYGroverSPMaqsoodAHoustonRAyCNoubouossieDFet al

Glutamine and glutamate--their central role in cell metabolism and function

Binders are used to hold all the ingredients together, so they retain the shape of a tablet