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5.3 Contrast-Induced (CI) AKI As the third most common cause of AKI, the use of contrast agents and contrast-induced nephropathy (CIN) have become the focus of global public health attention (Yang et al., 2015)

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This also means that poorly designed studies risk confounding readouts if timing and dosing windows are not aligned to each compounds mechanism

This can lead to a deficiency in Complex II of the mitochondrial electron transport chain, which generates reactive oxygen species (ROS)

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Materials and Methods Preparation of the riboflavin deficient medium The riboflavin deficient medium used in this study consisted of the following components: (1) 90% (by volume) riboflavin-free customized type B medium (three portions of -MEM and one portion of Waymouths MB 752/1) developed by Adeli et al