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Carbone AL, Moroni M, Groot-Kormelink PJ, Bermudez I (2009)

Co-Immunoprecipitation and Immunoblotting Analysis Cells were washed twice with phosphate-buffered saline (PBS), and then lysed in a lysis buffer on ice for 30 min, and then cell lysates were centrifuged at 12,500 rpm for 15 min

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Increased GSH levels and GCL expression occur in many tumors and have been implicated to confer drug and/or radiation resistance to tumors of ovarian, lung, prostate and colorectal origins (Lu, 1999a)