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Membranes were blocked with 5% non-fat milk and incubated with mouse anti-5mC (#61480, Active motif) or anti-5hmC (#39999, Active motif) followed by a horseradish peroxidase-conjugated antibody (Santa Cruz Biotechnology)

Jul 2021;39(3):391-398

[DOI] [PMC free article] [PubMed] [Google Scholar] 28.Gibbons CH, Wang N, Freeman R

Their diverse roles in synapse remodeling, trophic support, and neurotransmitter homeostasis indicate a potentially significant yet incompletely understood impact on synaptic signal alterations following RGC axon injury

Short-term risks include the potential for allergic reactions or infections at the injection site

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