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As before, an empty cage was placed at the narrow end of the beam and served as the goal for successful completion of the beam walk

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coli M4020, obtained by insertional disruption of the wsc genes required for colanic acid biosynthesis, was less tolerant to exposure to 55 and 60C than its parental strain E

Its important to rotate injection sites each time to avoid skin irritation and infections

Trichloroacetic acid (TCA, 10%) was added to the homogenate, the mixture was centrifuged at 3000 rpm for 5 min, and the supernatant was collected