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Microscopy was performed with confocal microscopy (Zeiss Axio Imager M2 with an Axiocam 712 mono camera) using Zen software (v3.4, Blue Edition) or automated cell imagers (Agilent, BioTek Lionheart FX automated microscope) using Gen5 software (v3.12)

Overall, SCFAs significantly impact regulatory T-cells and effector T-cells based on the immunological context, with intestinal T-cells being a major target due to elevated SCFAs levels in colonic tissues and gut-associated lymphoid tissues

[DOI] [PubMed] [Google Scholar] 137.Mueller S.G., Trabesinger A.H., Boesiger P., Wieser H.G

J Physiol Genomics

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J Bacteriol 89:12991305