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Cell extract containing hPrdx6 protein was collected after cell lysis using lysis buffer (20 mM Tris (pH 7.0), 50 mM NaCl, 10 mM Imidazole, 0.5 mg/ml lysozyme, 0.005 mg/ml RNaseA, 0.005 mg/ml DNase) and sonication (12 pulses of 30 s)

Vighi G, Marcucci F, Sensi L, Di Cara G, Frati F

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Appl Biochem Biotechnol 194(10):43774399

, : Joe Rogan

Microscopy revealed integration of muscle fibers into newly formed cortical bone, along with organized collagen deposition and periosteal reactivation
