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doi: 10.1038/s41580-020-00324-8 186 YeePPWeiYKimS-YLuTChihSYLawsonCet al

Vitamin B12 deficiency presenting as pancytopenia with ring sideroblasts

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After incubation for 4 h at 37 C, the steroids were extracted with chloroform, evaporated by centrifugal concentrator, and re-dissolved in methanol for liquid chromatographymass spectrometry (LCMS) analysis, using buffer A containing 1 mM ammonium formate and buffer B containing 100% methanol

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elegans AGO-clade Argonautes (5 AGOs) interact with miRNAs and 26G-RNAs, the PIWI clade (1 AGO) with 21U-RNAs (the worm piRNA ortholog), and the WAGO-clade (13 AGOs) with 22G-RNAs