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To evaluate the use of this enzyme in manipulating compartment-specific GSH pools, we transduced HEK 293T cells with either untargeted or mitochondria-targeted, human codon-optimized GshF , henceforth referred to respectively as GshF and mito-GshF , and confirmed their subcellular localization by cell fractionation (Fig

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138.Weimbs T, Saville J, Kalantar-Zadeh K

Under normal conditions, Keap1 complex-mediated ubiquitination degrades Nrf2, and when confronted with oxidative stress, Keap1 complex activity is inhibited and the Nrf2/ARE pathway is activated