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Membranes were washed in TBST, incubated with HRP-conjugated secondary antibodies for 90 min at room temperature, and developed using ECL regents followed by visualized using the Tanon 5200 chemical luminescence imaging system

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Glutamine enhances the transactivation of HSF1 and induces HSF1 expression via cytosine-cytosine-adenosine-adenosine-thymidine (CCAAT) box motif which is present in several gene promoters enhancer-binding protein- in a dependent manner

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Jasinski et al 1985

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