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For YNB medium transfer experiments, fresh overnight cells of each strain were then inoculated into the filtered spent medium (SM) and aliquoted into a 96-well plate at an OD 600 of 0.0001 and grown for 72 h at 30C with shaking at 220 rpm and OD 600 measurements taken every 24 h

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Small bubbles in the column should be removed by passing a de-gassed buffer

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Purification and analysis of biomolecules can be actualized based on their shape, particle size, charge, molecular structure and binding affinity to the stationary phase