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Antioxid Redox Signal 12: 743-785

The final hydroxylation step takes place in the renal proximal convoluted tubules, where the enzyme vitamin D-1-hydroxylase (CYP27B1) converts 25(OH)D into 1,25-dihydroxycholecalciferol (calcitriol), the biologically active form of vitamin D

After dissolving the pellet in urea (300 l, 8 m in 0.1 m TEAB buffer) via mild sonication (10%, 5 cycle, 10 s), the sample was centrifuged (13,000 g , r.t., 3 min)

Most evidence remains preclinical, and more research is needed to determine long-term consequences and optimize chronic therapy regimens

doi: 10.3389/fphys.2022.970292 24

Membrane Dialysis membrane with !2-l4kd molecular weight cut off