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MitoSOX Red indicator and PI were detected using the TRITC filter (Ex/Em: 396/610 nm) and Texas Red (Ex/Em:560/630), respectively, while Hoechst was detected using HOECHST 33342 filter (Ex/Em: 353/483)

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Knockdown of NNMT in adipocytes was associated with increased NAD+ levels and altered expression of genes involved in energy expenditure, providing the rationale for pharmacological NNMT inhibition [2]