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After all non-retained components are washed off the column, binding solute or together with ligand as solute-ligand complex are eluted by presence of mobile phase which was prepared in suitable pH, ionic strength and solvent composition for solute-ligand binding

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These spatial profiles confirmed the colocalization of -synuclein aggregates with astrocyte clusters expressing low SLC7A11 and high SAT1 and ACSL4, implicating localized iron dyshomeostasis in disease progression [171]

Intranasal and Localized Delivery : Localized routes such as the Minimally Invasive Nasal Depot (MIND) can bypass the BBB by delivering antibodies directly to the olfactory region for sustained brain release

doi: 10.1016/j.jveb.2022.03.002