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After 24 h, the cells were treated with either BSA control or BSA-conjugated palmitic acid (PA 200 M) overnight

Data availability Data available on request from the authors

2) For large volume of supernatant, concentrate the proteins by ammonium sulphate precipitation, dialyze it against 1 PBS at 4C before applying it onto the column

Role of B12 in Red Blood Cell Formation Vitamin B12 is vital for making red blood cells

4.3 Regulating the gut microbiota and immune system When the IEB is damaged, the permeability of the IEB increases, lipopolysaccharides and other endotoxins produced by bacteria can activate the peripheral immune system, activate immune cells and related signaling pathways, promote the release of inflammatory factors and cause systemic inflammation, and also promote the migration and infiltration of peripheral immune cells to the brain, leading to central nervous system inflammation through the blood-brain barrier (BBB) (Caspani and Swann, 2019)

(2) One patent pending: Lipidomic approaches for central nervous system disorders Application # 12/091,213 filed 12/10/08, Publication # US 2009/0305323 12/10/09.] All other authors declare no conflict of interest