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Antibodies The following primary antibodies were used: anti-GPX4 (#52455), anti-SLC7A11 (#12691), anti-phosphoSirT1 (Ser47) (#2314), anti-phosphoCDK2 (Thr160) (#2561), anti-PhosphoRB1 S807/811 (#8516), anti-cleaved PARP (#5625), anti-cleaved Casp3 (#9664), anti- Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (#4370), Anti- 5-Lipoxygenase (ALOX5) (#3289) from Cell Signaling Technology at a 1:1000 dilution

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(2009) cMyc suppression of miR-23a/b enhances mitochondrial glutaminase expression and glutamine metabolism, Nature , 458 , 762-765, doi: Altman, B

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All samples were embedded in paraffin and sectioned into 8 m slices

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