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Wang DZ, Wang C, Wu H, Li ZM, Ye Q (2016) Glutathione production by recombinant Escherichia coli expressing bifunctional glutathione synthetase

MTT assay was conducted using a microplate reader (SpectraMax)

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Cells were lysed with 500 L of RIPA lysis buffer containing 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 25 mM Tris-HCl, supplemented with protease and phosphatase inhibitors at a 1:100 dilution

doi: 10.1002/hep.23476