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doi: 10.2217/epi-2018-0187

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All secondary antibodies were diluted 1:200 in PBS with 1% goat serum and 0.1% Triton-X (Sigma-Aldrich)

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Add the elution buffer to the column, cap both ends, and incubate for a few minutes before uncapping and dripping your elution out of the bottom (Figure 4)

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