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PLoS ONE 4 : e4224

Dill DB, Horvath SM, Van Beaumont W, Gehlsen G, Burrus K

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Briefly, 10-fold diluted serum (100 l for each sample) was added to the wells of an OVA-coated ELISA plate (NuncMaxiSorp flat-bottom, Thermo Fisher Scientific), followed by addition of biotinylated rat anti-mouse IgE (R35-72, 2 g/ml, BD Biosciences), horseradish peroxidase-conjugated avidin (40-fold dilution, BD Biosciences), and NeA-Blue (tetramethylbenzidine substrate

Effects on offspring development occurred at doses below those that produced evidence of maternal toxicity in these studies

It does not recover