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The seed extract and its major bioactive constituent L-dopa were further studied to understand their neuroprotective potential against ochratoxin Ainduced oxidative stress via reactive oxygen species generation, mitochondrial membrane potential disruption, nuclear damage and neuronal stress in Neuro-2a cells

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To simplify purification, affinity purification tags are often fused to a recombinant protein of interest.The most popular tag is the polyhistidine (6xHis) tag.Some proteins, like eukaryotic proteins may not exhibit proper protein activity or folding if expressed in E.coli

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