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Induction of apoptosis was detected by Propidium Iodide (PI, 1 g/mL) (Sigma Aldrich) and Annexin-V-APC (Immunotools) staining, followed by flow cytometry analysis, using a BD FACSCantoII flow cytometer

doi: 10.1519/1533-4287(2003)017 2.0.co;2

The permeabiliy of natural membranes

In atrophying muscles, there is activation of the Forkhead box O transcription factor family (FOXO), as well as NF-B, which can be activated by TNF-, soluble TNF-like weak inducer of apoptosis (TWEAK), or IL-1 [15, 16]

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Meanwhile, the lipogenic genes SREBP-1c, DGAT2, FAS and ACC1 can be activated by the overexpression of the direct lipogenesis regulator miR-122 through the effect of miR-370