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To avoid the unspecific cleavage of APC-specific substrate by thrombin, residual activity of thrombin was inhibited by addition of recombinant hirudin (Refludan, Bayer Health Care)

Footnotes Appendix A

doi: 10.1016/j.bbagen.2022.130239 79 ErdamarHCimenBGlcemalHSaraymenRYererBDemirciH

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This spike in ROS levels has various negative impacts on tissue