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First, the parent compound NdFeAsO was grown on MgO(001) at 800 C, followed by the deposition of a NdOF over-layer at the same temperature, from which fluorine diffused into the NdFeAsO layer 48,49,53,54

This established Leydig cell model allowed for controlled induction of senescence and testing of FOXO4-DRI effects

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10.2147/CCID.S397751 113

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Opto-genetic manipulations An optic fiber cannula for micro dialysis and LED cannula for behavioral testing were placed targeting the dorsal raphe nucleus (DRN)