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HGF also reduces the number of astrocytes by retaining the levels of the glial-specific glutamate transporter 1, thus favoring a reduction in glutamatergic neurotoxicity (Sun et al., 2002)

The half-life of 159 to 195 hours means there is some flexibility, a day early or late will not ruin the protocol, but regular weekly timing produces the most stable plasma levels

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This increase implies that these miRNAs have significant regulatory functions throughout the early stages of embryonic development and during the activation of the genome [219, 221], Early bovine blastocysts showed increased expression of miR-135a, miR-218, miR-335, and miR-449b in comparison to hatched ones [264], The mouse embryos dynamic and strictly controlled expression patterns of miRNAs throughout preimplantation development are best shown by the downregulation of miR-182 between the 2- and 4-cell stages and the subsequent increase between the 4- and 8-cell stages

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