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In addition, these methods should be combined with conventional techniques, such as sperm chromatin structure assay, computer-assisted sperm analyses (CASA), integrity of membranes, flow cytometry, and reactive oxidation stress levels, to determine semen quality using system biology approaches

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Studies have shown that dietary supplementation with arginine can promote the synthesis of Ca 2+ and cAMP (Cyclic adenosine monophosphate), thereby improving semen quality in boars (Dong et al., 2018)