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Description

( Table 2 )

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Nature 375 , 812815 (1995)

Contraindications Do not take if hypersensitive to any of the ingredients

The design principle: Standard (often fails): His6 - TEV site - Target protein Better: His6 - TEV site - (GGS)2 - Target protein Even better: His6 - (GGS)2 - TEV site - (GGS)2 - Target protein Failure 2: Non-Specific Cleavage (The Protease Damages Your Protein) The problem: Proteases aren't perfectly specific