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A recent study found that coculturing exosomes from PC3 cells in estrogen receptors with macrophages activated by THP-1 causes macrophages to polarize into the M2-type, which is characterized by high programmed death-ligand 1 (PD-L1) expression and IL-6 and IL-10 release [112]

7 Pickering G, Schneider E, Papet I, Pujos-Guillot E, Pereira B, Simen E et al (2011) Acetaminophen metabolism after major surgery: a greater challenge with increasing age

The Bradford assay was used to determine protein concentration of cell extracts, from there samples with the same amount of protein were separated using SDS-PAGE technology and transferred onto a PVDF membrane

doi: 10.1186/s13550-017-0301-4 14 AskewK.LiK.Olmos-AlonsoA.Garcia-MorenoF.LiangY.RichardsonP.et al

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It has been assigned Generally Recognized As Safe (GRAS) status by the FDA for food, beverage, and dietary supplement applications
