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Tetracycline (3 g mL 1 ) and spectinomycin (100 g mL 1 ) were used to select plasmid-harboring transformants

b- MTHFR activity, B12 vitamin and male infertility Many studies have linked MTHFR T/T (homozygous mutation) or C/T (heterozygous mutation) as well as double heterozygote MTHFR 677 C/T and 1298 A/C genotypes with: Low sperm count Low sperm motility Male infertility Folate deficiency has been correlated with: sperm aneuploidy (4) sperm DNA damage (5) low sperm count (6) While total homocysteine (tHcy) concentrations in seminal plasma has been linked to: male subfertility low embryo quality (7)

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Correlative organelle fluorescence microscopy and synchrotron X-ray chemical element imaging in single cells