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Biguanide-mediated ether lipid synthesis is necessary for a pro-longevity benefit irrespective of bacterial growth or metabolism Previous studies into the biological action of metformin have suggested that biguanides mediate their lifespan-extending properties in the nematode through alterations in growth and metabolism of their bacterial food source (Cabreiro et al., 2013

[DOI] [PMC free article] [PubMed] [Google Scholar] 261.Sorensen, J

Therefore, AX reactivity and conjugation to carrier molecules have been the subject of thorough investigation ( Initial assays preincubating proteins with reducing agents before treatment with AX showed a clear decrease in AX-protein adduct formation in the presence of DTT, whereas the effect of GSH was less consistent ( Figure 1 )

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The production of ROS is linked to the overexpression of a misfolded disulphidebonded protein, carboxypeptidase Y (CPY * )

The combination targets different aspects of the problem