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The seller tone helps, like break up everything afterwards and I kind of made up the transitions a little bit smoother and it was

10.1039/c1ob05996c 17 BorhadeS

The tubes were vortexed and incubated in the ultrasonic bath for 10 min

To limit glial scar formation, a cocktail of three mitotic inhibitors was added to the medium for the first 3 days of culturing: uridine, 5-fluorodeoxyuridine and cytosine--arabinofuranoside [4.4 mM each, Sigma, #U3003, #F0503 and #C1768]

doi: 10.1002/alz.083029 117 MollenhauerB.Caspell-GarciaC.CoffeyC

Similarly, we anticipate that spatial mapping will identify zones of high GPX4 expressionestablished by Li et al