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Strategies to minimize reactions include rotating injection sites consistently to avoid repeated trauma to the same area, ensuring the solution is at room temperature before injection (cold injections can increase irritation), injecting slowly rather than quickly, and using a smaller gauge needle for less tissue trauma

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Intriguingly, quantification of the numbers of neurons in the myenteric plexus of germ-free versus SPF mice revealed a significant reduction in numbers in the duodenum and ileum but not in the colon, which seems somewhat counterintuitive and not readily explicable to this point

In this article, we'll explore the v

But what we do have is encouraging

PMC 6337226