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Neutral Red uptake assay (above): NHEKs were plated into 48-well culture plates (18,000 cells per well) and allowed to recover for two days in a serum-free medium

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made use of the RPTEC/TERT1 cell line to evaluate transepithelial transports in a small set of known nephrotoxic and non-nephrotoxic drugs, demonstrating that further evaluation of functional parameters, including transepithelial electrical resistance (TEER), reabsorption, and secretion of solutes, is essential to understand the nephrotoxicity mechanisms of drugs and predict the in vivo implication in regards to kidney performance (Secker et al

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