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Importantly, the mechanisms of toxicity in mice are very similar to humans [125, 126]

The free radical then reacts with an oxygen molecule to generate a corresponding peroxy radical, which captures the allyl hydrogen of another molecule and converts it into a hydroperoxide

Antioxidant and pro-oxidant effects

This fragment was digested with EcoRI (NEB) and ligated into pGex-6p1 (GE Healthcare) prepared by digestion with BamHI and blunting with Mung Bean Nuclease (NEB) to generate pJB-GST-HTS

This information is significant in the context of the simultaneous administration of the Epidiolex product (synthetic cannabidiol: GW Pharmaceuticals) as an adjuvant in patients with refractory epilepsy treated with clobazam, which is another substrate for these enzymes that belong to the CYP family