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Immune, primarily antigen-presenting cells of myeloid origin as well as nonimmune cells (Qu et al., 2017) perceive extracellular HSP70 signaling via either C-type lectin or scavenger pattern recognition receptors (PRRs), thereby initiating cytokine release involved in innate immune responses ( Non-immune cell interaction Moreover, extracellularly released HSP70 exerts signaling activities beyond the immune system

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These proteins are concentrated in the serum, which is collected after 24 hours and can be frozen and stored for future use

DSIP/Selank can often be safely combined with other sleep and stress wellness approaches including cognitive behavioral therapy for insomnia (CBT-I) which addresses behavioral sleep patterns, mindfulness-based stress reduction (MBSR) and meditation practices, comprehensive sleep hygiene optimization, other recovery-supporting peptides, magnesium and sleep-supporting supplements, adaptogenic herbs like ashwagandha or rhodiola, and comprehensive nervous system optimization protocols when properly coordinated under medical supervision