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Western blotting Cells lysates were collected in 1X sample buffer (2% SDS, 10% glycerol, 0.01% bromophenol blue, 62.5mM Tris, pH 6.8, 0.1M DTT) and boiled to 95C for 10 min

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However, the best definition of an antioxidant was proposed by Halliwell and colleagues in 1995
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doi: 10.1038/s41577-024-01026-4 9 LeoneRDPowellJD

Blood levels of thiamine and ascorbic acid in chronic open-angle glaucoma

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