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Real-time qPCR was performed using an Applied Biosystems QuantStudio 12 K Flex real time PCR system (Thermo Fisher Scientific, San Jose, CA, USA) with THUNDERBIRD SYBR qPCR Mix (Toyobo), 6 pmol of primers, and aliquots of cDNA

Studies have demonstrated that FOXO4-DRI modulates multiple regulatory pathways, including insulin signaling, cell cycle control, and oxidative stress response

Acta Pharmacol Sin 2000

10.1097/SLA.0000000000000287 44 LavergneS

It follows the same regulatory arc as BPC-157 and TB-500: Category 2 placement in 2023, removal from Category 2 in April 2026, and formal PCAC review scheduled for July 23, 2026