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Description
The purified proteins were eluted using elution buffers containing reduced glutathione (10 mM) or imidazole (250 mM), respectively

EdU (RiboBio, Guangzhou, China) labeling solution (1:1,000 dilution) was added and incubated for 2 h

It was found that equilibrium had always been reached by the time the first spectrum was measured (1020 min after mixing)

If prolonged erections occur, a second injection at a medical office can reverse it

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For example, liver X receptor agonists promote cholesterol efflux to enhance TCR signaling [385]
