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The second mechanism is characterized by the modulation of intracellular calcium, which seems to function as a trigger for rapid insulin exocytosis and complements the amplification phenomena [8]
1) in the journal eLife

Without these, the COA should be considered incomplete
10.1074/jbc.M114.549634 J
Oaklander, A

Mechanized platforms for automated liquid handling, high-throughput screening, and real-time cell analysis now allow for more scalable and reproducible screens