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Although recent advances in the study of Mn biology have revolutionized our understanding of its physiological roles, several critical knowledge gaps remain

The 2 CT method was used to calculate relative changes in gene expression determined from qRT-PCR experiments (Wai et al., 2020)

A 3-month RCT of NAC, 1200 mg per day, versus vitamin C, 2000 mg per day, found significant improvements in ALT and spleen span, but not other liver enzymes or liver span with NAC, whilst improvements in steatosis grade failed to reach statistical significance

10.1038/s41580-019-0190-7 Nat Rev Mol Cell Biol

Lipomax reagent was used for transfection (LipoMax32012, Sudgen), and correct expression was validated by Western blotting

Thermo Fisher Scientific) for buffer exchange with buffer A (supplemented with 1 mM DTT)