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Stage-tips were activated with MeOH (2 60 L), washed with Buffer B (80% CH 3 CN, 20% water, 0.1% formic acid) (1 60 L), and equilibrated with Buffer A (2 60 L)

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Indeed, in kidney marrows from young-adult fish, the fraction of cells in the S/G2/M phase was higher compared to aged fish, supporting the higher proliferation signal in immune cells isolated from young-adult fish (Extended Data Fig