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IDA binds to the activated agarose through the reactive epoxy group and chelates Ni 2+ ions to interact with his-tagged proteins (Figure 5)

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The process depends on the type of drug and reaction

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otherwise, it may reduce the affinity and change the conformation of the proteins, preventing the target protein to bind to the residues as expected

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Specifically, Glu58 on H2 provides a major symmetric polar interaction with Gln53 on H2 of opposite monomer (Supplementary Fig

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2012;120(21):4018.22990017 [Google Scholar] 167

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Molecules 27(5):1620

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