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The eluted proteins were concentrated using a 100 kDa cutoff centrifugal filter (Millipore) and subjected to size exclusion chromatography (SEC) on a Superose 6 Increase 10/300 GL column (Cytiva) pre-equilibrated by buffer A 1 supplemented with 6 mM MgCl 2

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Mistake 6: Improper storage temperature Leaving the reconstituted vial on your bathroom counter