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This validation process ensures that observed interactions are genuine and not artifacts of the experimental conditions

The serum separation tubes were allowed to stand at room temperature for 15 min, centrifuged for 10 min, and serum was removed and placed into a microfuge tube

doi: 10.1089/ars.2011.4037 49 MicalettiF.EscoffreJ

The glutathione model found that all glutathione would be converted to glycine in 548.6 seconds

The protein expression and activity assays of the D23A mutant were performed as described for the U46C mutant

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