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The hippocampal tissue was disrupted using a T10 Ultraturrax homogenizer (IKA Werke GmbH) followed by RNA extraction with TRIzol Reagent (Invitrogen, Thermo Fisher Scientific) according to the manufacturers instructions

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It has been shown to help lower blood sugar levels and promote cartilage healing without increasing long bone growth, increasing IGF-1 levels, or altering insulin sensitivity